ZAG DNA Analyzer

The ZAG is the ideal instrument for large-scale, high-throughput facilities focused on the qualitative assessment of DNA fragments.

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What is the ZAG DNA Analyzer?

The ZAG DNA Analyzer is a high throughput capillary electrophoresis instrument designed for the qualitative analysis of DNA fragments. Ideal for facilities that must screen thousands of DNA fragments per day, the ZAG relieves analytic bottlenecks with an easy to use protocol and intuitive analysis software.

A specialized software package, PROSize® Data Analysis Software, enables the quick analysis of DNA fragments post-capillary electrophoresis. Capable of the simultaneous analysis of thousands of separations, PROSize directly enables the quick and efficient assessment of peaks of interest.

High Separation Resolution
Reliably distinguish and size fragments within 3 bp in as little as 15 minutes.
High Throughput
Large scale DNA fragment analysis has never been easier. Process over 48, 96-well sample trays in 24 hours.
Broad Sizing Range
Size fragments between 1 bp and 20,000 bp through a variety of available DNA analysis kits.

How does the ZAG DNA Analyzer reduce analytic bottlenecks?

DNA fragment analysis is a crucial procedure in many molecular biology laboratories. In high-throughput molecular biology settings, analysis of DNA fragments often poses a bottleneck as conventional analytic methods, such as agarose gel electrophoresis, do not scale up well. The ZAG™ DNA Analyzer alleviates these DNA fragment analysis bottlenecks allowing labs to quickly and accurately perform fragment analysis on DNA samples up to 20,000 bp in size.

Flexible enough to handle any workflow senarios

The ZAG provides superior DNA fragment analysis to high throughput molecular biology laboratories.
Separate 96 samples in parallel and achieve 3 bp resolution with AATI's custom 96 capillary array.
Load nine, 96-well sample trays at a time to separate up to 864 samples without researcher intervention.
Load two different gels to set up diverse separation runs.

PROSize® Data Analysis Software

As a high-throughput instrument the ZAG DNA Analyzer produces large volumes of data, posing a challenge to researchers. PROSize Data Analysis Software ameliorates data analysis challenges posed by the ZAG with Batch Processing, assessing hundreds of runs at once. Coupling Batch Processing with Flag Analysis or Advanced Flag Analysis allows PROSize to automatically mark samples of interest for researchers.

Applications

The ZAG is well suited to the high-throughput analysis of DNA fragments for various applications, from PCR amplicon analysis to basic genotyping protocols.

PCR Fragments

Determination of amplicon size and concentration is a crucial aspect in post-reaction analysis of PCR. Traditional methods of PCR fragment analysis lack the sensitivity and resolution demanded by many applications.

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Genetic Markers

Genetic markers allow for the simple identification of genetic variants, providing crucial information to researchers. The qualitative family of kits for the ZAG provide the necessary tools to improve genetic marker identification workflows.

Commonly analyzed nucleic acid samples include:

  • SSR/Microsatellites
  • PCR Amplicons
  • Restriction Enzyme Digests

Technical Information

Technology

Capillary arrays are the core of the, ZAG™ DNA Analyzer, affecting all aspects of electrophoresis. Robust capillary array cartridges are a defining trait of the ZAG DNA Analyzer, shortening time to results by reducing the amount of conditioning steps necessary between separation runs, requiring only a single conditioning step for multiple runs, while maintaining resolution and quality.

Diagram Parallel Capillary Electrophoresis Array

dsDNA samples are loaded into the capillaries via electrokinetic injection. After injection, constant voltage is applied to separate DNA fragments. Capillaries pass through the detection window, where the DNA fragments are quantified in real-time by the optical platform.

Nucleic acid detection is accomplished with a rigorously designed optical platform comprised of an LED light source (470 nm excitation), camera lens, and CCD detector. Fluorescent light passes from the LED through the array window. The emitted fluorescence from the intercalating dye then passes through the camera lens and specific band pass filters and spatially imaged onto the CCD detector. This system enables real-time detection of nucleic acid separations.

Specifications

Maximum Capacity Nine 96-well sample trays or ~48 96-well plates/day (ZAG-135 Kit)
Maximum Unattended Sample Capacity 864 samples
Minimum Sample Volume 20 µL of liquid for injection; direct injection of PCR samples possible
Resolution Gel Dependent; as low as 3 bp
Sizing Accuracy Typically 5% or better
Detection Sensitivity As low as 5 fg/µL for fragments
Light Source 700 mA, 10 W LED, 470 nm excitation wavelength
Emission Collection Device CCD Detector
Software ZAG™ Instrument Control Operational Software, PROSize® Data Analysis Software
Data Export Format CSV, PDF, flexible numerical or binary output options
Environmental Conditions Indoor use, normal laboratory environment 20-23°C
Relative Humidity Range <80% (non-condensing)
Electrical 100-200 VAC; 50-60 Hz
Instrument Dimensions 82 cm H x 63 cm W x 59 cm D (33 x 25 x 23 in)
Instrument Weight 68 Kg (150 lbs)

Reagent Kits

The reagent kits below are designed for the high-throughput sizing and relative quantification of dsDNA fragments.

(ZAG-105) ZAG 105 dsDNA Kit

Sizing Range: 1 bp – 500 bp

(ZAG-110) ZAG 110 dsDNA Kit

Sizing Range: 35 bp – 5,000 bp

(ZAG-130) ZAG 130 dsDNA Kit

Sizing Range: 75 bp – 20,000 bp

(ZAG-135) ZAG 135 dsDNA Kit

Sizing Range: 1 bp – 1,500 bp

Miscellaneous Parts and Components (contact your sales person for more details)

  • (A1300-033) Modified Bottle Cap for 50mL Conical Tube
  • (A1300-034) Modified Bottle Cap for 250mL Conical Tube
  • (A1300-036) Buffer/Waste Tray Carrier
  • (M1300-025) Tray Carrier - Standard
  • (F1300-109) Tray Carrier - Fully Skirted Tray
  • (F1300-107) Tray Carrier - 12 vial strip (0.2 mL)
  • (P60-20) Fragment Analyzer 96-Well Buffer/Waste Tray
  • (P60-22) Fragment Analyzer Waste Tub
  • (FS-SMO15) Mineral Oil Dropper Bottle, 15mL
  • (GP-440-0100) Capillary Storage Solution, 100 mL

Capillary Arrays

Capillary arrays are available in two conformations for the ZAG™ DNA Analyzer:

Array length is provided in centimeters with two numbers. The first number is the effective length of the capillaries – the region of the capillary observed for separation – while the second number describes the overall length of the length of the capillary from end to end. The number of capillaries in each array directly impacts throughput, the more capillaries per array, the more samples that can be run in parallel. Array length affects both the speed of the separation and the separation resolution. A shorter array will provide faster electrophoresis times, while a longer array will increase separation resolution.

Use the ZAG capillary array-kit matrix below (separation times provided in minutes) to identify which capillary array is best suited for each unique application and level of throughput.

ZAG-105 ZAG-110 ZAG-130 ZAG-135
96 Capillary Short 60 50 30 20
96 Capillary Long 80 80 75 35

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