Reagent Kits

Our capillary electrophoresis instruments utilize specially designed reagent kits to analyze diverse nucleic acid samples, from small RNA to genomic DNA, and NGS library quality control.

Reagent Kit Box

ZAG 135 dsDNA Kit (1-1500bp)

The ZAG 135 dsDNA Kit (1-1500bp) (ZAG-135-5000) is ideal for rapid qualitative separations of dsDNA fragments on the ZAG DNA Analyzer. Obtain relative quantification and accurate sizing of dsDNA fragments between 1 bp and 1,500 bp in as little as 20 minutes.

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Components

5,000 Sample Kit
ZAG 130 dsDNA Separation Gel 500 mL (ZAG-130-0500)
Intercalating Dye 30 µL X 2 (DNF-600-U030)
5X 930 dsDNA Inlet Buffer 500 mL (DNF-355-0500)
5X Capillary Conditioning Solution 100 mL (DNF-475-0100)
1 bp and 1,500 bp Markers, 10% Formamide 3.2 mL (FA-MRK910F-0003)
100 bp DNA Ladder 100 µL X 2 (FS-SLR910-0001)
Mineral Oil Dropper Bottle 15 mL (FS-SMO15)
Dilution Buffer 1X TE 125 mL (DNF-495-0125)

Specifications

Specifications Description
Sample Volume Required 2 µL (adjustable depending upon sample concentration)
Total Electrophoresis Run Time 20 minutes (33-55 Array); 35 minutes (55-80 Array)
DNA Sizing Range 100 bp – 1,500 bp
Separation Resolution 1 bp – 100 bp ≤ 15%; 100 bp – 1,500 bp ≤ 10% (33-55 Array); 1 bp – 1,500 bp ≤ 5% (55-80)
DNA Sizing Accuracy ±5% or better
DNA Sizing Precision 5% CV
DNA Fragment Concentration Range 0.5 ng/µL – 50 ng/µL input DNA (adjustable by dilution of sample)

Examples

The ZAG 135 dsDNA Kit (1-1500bp) (ZAG-135-5000) enables the fast, high-throughput qualitative analysis of DNA fragments up to 1,500 bp in length. Separations are performed in as little as 20 minutes, requiring the use of a specially designed DNA ladder that maintains the necessary fragment resolution for DNA fragment sizing under more intensive separation conditions. The 100 bp DNA Ladder (Figure 1) accomplishes this, enabling sizing of DNA fragments up to 1,500 bp, ideal for PCR amplicon analysis, genotyping, and microsatellite detection.

Figure 1
Figure 1. Separation of the 100 bp DNA Ladder (FS-SLR910-0001) performed on a ZAG DNA Analyzer equipped with a Short Capillary Array (33-55) using the ZAG 135 dsDNA Kit (1-1500bp) (ZAG-135-5000).
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