Reagent Kits

AATI's capillary electrophoresis instruments utilize specially designed reagent kits to analyze diverse nucleic acid samples, from small RNA to genomic DNA, and NGS library quality control.

Reagent Kit Box

ZAG 130 dsDNA Kit

ZAG 130 dsDNA Kit
ZAG 130 dsDNA Kit

The ZAG 130 dsDNA Kit enables the separation of dsDNA fragments across a broad sizing range. DNA fragments are sized between 75 bp and 20,000 bp in as little as 30 minutes without sacrificing resolution. Common applications include the qualification of PCR fragments, genotyping, and microsatellite identification.

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Components

5,000 Sample Kit
dsDNA Gel 500 mL (ZAG-130-0500)
Intercalating Dye 30 µL X 2 (DNF-600-U030)
5X 930 dsDNA Inlet Buffer 500 mL (DNF-355-0500)
5X Capillary Conditioning Solution 100 mL (DNF-475-0100)
75 bp and 20,000 bp Markers 3.2 mL (FS-SMK930-0003)
1,000 bp Plus DNA Ladder 100 µL X 2 (FS-SLR930-U100)
Mineral Oil 15 mL (FS-SMO15)
Dilution Buffer 1X TE 125 mL (DNF-495-0125)
Conical 250 mL Centrifuge Tube

Specifications

Specifications Description
Sample Volume Required 2 µL (adjustable depending upon sample concentration)
Total Electrophoresis Run Time 30 minutes (33-55 Array); 75 minutes (55-80 Array)
DNA Sizing Range 75 bp – 20,000 bp (defined by lower/upper marker)
Separation Resolution 75 bp – 1,500 bp ≤ 10%; 1,500 bp -20,000 bp ≤ 15%
DNA Sizing Accuracy ±10% or better
DNA Sizing Precision 5% CV
DNA Fragment Concentration Range 0.5 ng/µL – 50 ng/µL input DNA (adjustable by dilution of sample)

Examples

The ZAG 130 dsDNA Kit (ZAG-130-5000) provides rapid separation of large DNA fragments, making it well suited to the qualitative analysis of large PCR amplicons. The 1000 bp Plus DNA Ladder (Figure 1) promotes the quick qualitative analysis of large DNA fragments up to 20,000 bp in size through the careful selection of ladder fragments.

Figure 1
Figure 1.Separation of the 1000 bp Plus DNA Ladder (FS-SLR930-U100) performed on a ZAG DNA Analyzer equipped with a Short Capillary Array (33-55) using the ZAG 130 dsDNA Kit (ZAG-130-5000).
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