Reagent Kits

AATI's capillary electrophoresis instruments utilize specially designed reagent kits to analyze diverse nucleic acid samples, from small RNA to genomic DNA, and NGS library quality control.

Reagent Kit Box

ZAG 105 dsDNA Kit

ZAG 105 dsDNA Kit
ZAG 105 dsDNA Kit

The ZAG 105 dsDNA Kit provides a high-throughput, high-volume analysis solution for dsDNA fragments between 1 bp and 500 bp. Common applications of this kit include PCR amplicon sizing and genotyping.

Back to Kit List

Components

5,000 Sample Kit
ZAG 105 dsDNA Separation Gel 500 mL (ZAG-105-0500)
Intercalating Dye 30 µL X 2 (DNF-600-U030)
5X 930 dsDNA Inlet Buffer 500 mL (DNF-355-0500)
5X Capillary Conditioning Solution 100 mL (DNF-475-0100)
1 bp and 500 bp Markers, 10% Formamide 3.2 mL (FA-MRK900F-0003)
35 bp – 400 bp Range DNA Ladder 1.0 Ml X 2 (FS-SLR905-0001)
Mineral Oil 15 mL (FS-SMO15)
Dilution Buffer 1X TE 125 mL (DNF-495-0125)

Specifications

Specifications Description
Sample Volume Required 2 µL
Total Electrophoresis Run Time 60 minutes (33-55 Array); 80 minutes (55-80)
DNA Sizing Range 35 bp – 500 bp
Separation Resolution 5-10 bp @ 300 bp (33-55 Array); 3-5 bp @ 300 bp (55-80 Array)
DNA Sizing Accuracy ±5% or better
DNA Sizing Precision 2% CV
DNA Fragment Concentration Range 0.5 ng/µL – 50 ng/µL input DNA (adjustable by dilution of sample)

Examples

The high-throughput qualitative analysis of DNA fragments with capillary electrophoresis necessitates the use of a high quality DNA ladder. The 35 bp – 400 bp Range DNA Ladder (Figure 1) enables relative quantification and accurate sizing of DNA fragments between 1 bp and 500 bp (bounded by upper and lower markers). PCR amplicons, microsatellite, and genotyping applications are ideal uses of this kit.

Figure 1
Figure 1. Separation of the 35 bp - 400 bp Range DNA Ladder (FS-SLR905-0001) performed on a ZAG DNA Analyzer equipped with a Short Capillary Array (33-55) using the ZAG 105 dsDNA Kit (ZAG-105-5000).
Top