Reagent Kits

Our capillary electrophoresis instruments utilize specially designed reagent kits to analyze diverse nucleic acid samples, from small RNA to genomic DNA, and NGS library quality control.

Reagent Kit Box

dsDNA Reagent Kit (1-1500bp)

The dsDNA Reagent Kit (1-1500bp) (DNF-935) is a qualitative kit for the automated sizing and relative quantification of DNA fragments between 35 bp and 1,500 bp. Notably, this kit performs a separation in as little as 15 minutes, perfect for time-sensitive workflows. Common applications of this kit include genotyping, the analysis of PCR amplicons, microsatellites, and SSRs. Note: This is a qualitative kit for the accurate sizing and relative quantification of fragments.

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Components

Components500 Sample Kit1,000 Sample Kit
dsDNA 930 Gel240 mL (DNF-930-0240)500 mL (DNF-930-0500)
Intercalating Dye30 µL (DNF-600-U030)30 µL x 2 (DNF-600-U030)
5X 930 dsDNA Inlet Buffer125 mL (DNF-355-0125)300 mL (DNF-355-0300)
5X Capillary Conditioning Solution50 mL (DNF-475-0050)100 mL (DNF-475-0100)
1 bp and 1,500 bp Markers3.2 mL (FA-MRK910F-0003)3.2 mL (FA-MRK910F-0003)
100 bp DNA Ladder1.0 mL (FS-SLR910-0001)1.0 mL x 2 (FS-SLR910-0001)
Mineral Oil Dropper Bottle15 mL (FS-SMO15)15 mL (FS-SMO15)
Dilution Buffer 1X TE60 mL (DNF-495-0060)125 mL (DNF-495-0125)

Specifications

SpecificationsDescription
Sample Volume Required2 µL
Diluent Marker Volume Required22 µL
DNA Sizing Range100 bp – 1,500 bp (defined by lower/upper marker)
Separation ResolutionUltra-Short Array: 1 bp – 100 bp ≤ 15%; 100 bp – 1,500 bp ≤ 10%;
Short Array: 1 bp – 100 bp ≤ 15%; 100 bp – 1,500 bp ≤ 10%;
Long Array: 1 bp – 1,500 bp ≤ 5%
DNA Sizing Accuracy± 5% or better
DNA Sizing Precision2% CV
DNA Fragment Concentration Range0.5 ng/µL – 50 ng/µL input DNA
Total Electrophoresis Run Time15 min (Ultra-Short Array, 22-47); 20 min (Short Array, 33-55); 35 min (Long Array, 55-80)

Examples

Qualitative analysis of DNA necessitates the use of a well separated, quality ladder for the sizing of samples. The 100 bp DNA Ladder (FS-SLR910-0001) provides accurate and precise sizing via carefully selected DNA fragments and markers. The ladder enables the efficient and accurate qualitative analysis of DNA fragments including, PCR amplicons, microsatellites, and genotyping applications.

Figure 1
Figure 1. A separation of the 100 bp DNA Ladder (FS-SLR910-0001) carried out on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the dsDNA Reagent Kit (1-1500bp) (DNF-935).

Rapid and accurate separation of up to 96 samples in parallel is possible, providing excellent resolution in the separation of single or multiple fragments, as shown in Figure 2.

Figure 2
Figure 2. Digital gel image of a full 96 well plate of microsatellite samples. Electrophoresis was performed using the dsDNA Reagent Kit (1-1500bp) (DNF-935) with a Fragment Analyzer equipped with a Short Capillary Array (33-55).

A single microsatellite amplicon is shown in Figure 3 with an electropherogram and the accompanying digital gel image.

Figure 3
Figure 3. Separation of a single SSR amplicon using the dsDNA Reagent Kit (1-1500bp) (DNF-935). Capillary electrophoresis was performed using a Fragment Analyzer equipped with a Short Capillary Array (33-55).

Figure 4 depicts the electrophoresis of two microsatellite amplicons, distinct peaks visible on the electropherogram and the digital gel image.

Figure 4
Figure 4. Separation of two SSR amplicons using the dsDNA Reagent Kit (1-1500bp) (DNF-935). Capillary electrophoresis was performed using a Fragment Analyzer equipped with a Short Capillary Array (33-55).

As the example separation examples indicate, the dsDNA Reagent Kit (1-1500bp) (DNF-935) has the requisite sensitivity and accuracy for a wide variety of applications including genotyping while putting results in the hands of researchers faster.

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