Reagent Kits

Our capillary electrophoresis instruments utilize specially designed reagent kits to analyze diverse nucleic acid samples, from small RNA to genomic DNA, and NGS library quality control.

Reagent Kit Box

dsDNA Reagent Kit (75-20000bp)

The dsDNA Reagent Kit (75-20000bp) (DNF-930) is a qualitative kit for the accurate sizing of DNA fragments between 75 bp and 20,000 bp. Common applications of this kit include genotyping, the analysis of PCR amplicons, microsatellites, and SSRs. Note: This is a qualitative kit for the accurate sizing and relative quantification of fragments.

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Components

Components500 Sample Kit1,000 Sample Kit
dsDNA 930 Gel240 mL (DNF-930-0240)500 mL (DNF-930-0500)
Intercalating Dye30 µL (DNF-600-U030)30 µL x 2 (DNF-600-U030)
5X 930 dsDNA Inlet Buffer125 mL (DNF-355-0125)300 mL (DNF-355-0300)
5X Capillary Conditioning Solution50 mL (DNF-475-0050)100 mL (DNF-475-0100)
75 bp and 20,000 bp markers3.2 mL (FS-SMK930-0003)3.2 mL (FS-SMK930-0003)
1,000 bp Plus DNA Ladder100 µL (FS-SLR930-U100)100 µL x 2 (FS-SLR930-U100)
Mineral Oil Dropper Bottle15 mL (FS-SMO15)15 mL (FS-SMO15)
Dilution Buffer 1X TE60 mL (DNF-495-0060)125 mL (DNF-495-0125)

Specifications

SpecificationsDescription
Sample Volume Required2 µL
Diluent Marker Volume Required22 µL
DNA Sizing Range75 bp – 20,000 bp (defined by lower/upper marker)
Separation ResolutionShort Array: 75 bp – 1,500 bp ≤ 10%; 1,500 bp – 20,000 bp ≤ 15%
DNA Sizing Accuracy± 10% or better
DNA Sizing Precision5% CV
Maximum gDNA Concentration 0.5 ng/µL – 50 ng/µL input DNA
Total Electrophoresis Run Time 18 min (Ultra-Short Array, 22-47); 30 minutes (Short Array, 33-55); 75 minutes (Long Array, 55-80)

Examples

Qualitative DNA analysis necessitates the use of a well separated, quality DNA ladder for sizing samples. The 1000 bp Plus DNA Ladder provides accurate sizing via the use of high quality DNA fragments and markers. The ladder enables the efficient and accurate qualitative analysis of PCR amplicons, microsatellites, and genotyping applications.

Figure 1
Figure 1. A separation of the 1000 bp Plus DNA Ladder carried out on a Fragment Analyzier equipped with a Short Capillary Array (33-55) using the dsDNA Reagent Kit (75-20000bp) (DNF-930).

The dsDNA Reagent Kit (75-20000bp) provides true sizing of DNA fragments across a wide range, from 75 bp through 20,000 bp. Relative quantification of fragment concentration enhances the versatility of this kit. Easily perform genotyping, analyze PCR amplicons or microsatellites, among other applications. Figure 2 depicts the separation of DNA fragments across a wide range, from 115 bp to 14.7 Kb, using the dsDNA Reagent Kit (75-20000bp) (DNF-930).

Figure 2
Figure 2. Separation of seven DNA fragments of widely varying size using the dsDNA Reagent Kit (75-20000bp) (DNF-930). Electrophoresis was performed using a Fragment Analyzer equipped with a Short Capillary Array (33-55).
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