Reagent Kits

AATI's capillary electrophoresis instruments utilize specially designed reagent kits to analyze diverse nucleic acid samples, from small RNA to genomic DNA, and NGS library quality control.

Reagent Kit Box

dsDNA 910 Reagent Kit

dsDNA 910 Reagent Kit
500 Sample dsDNA 910 Reagent Kit

The dsDNA 910 Reagent Kit is a qualitative kit for the automated sizing of DNA fragments between 35 bp and 1,500 bp. Common applications of this kit include genotyping, the analysis of PCR amplicons, microsatellites, and SSRs. This is the recommended kit for the sizing of amplicons. Note: This is a qualitative kit for the accurate sizing and relative quantification of fragments.

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Components

Components 500 Sample Kit 1,000 Sample Kit
dsDNA 810 Separation Gel 240 mL (DNF-810-0240) 500 mL (DNF-810-0500)
Intercalating Dye 30 µL (DNF-600-U030) 30 µL x 2 (DNF-600-U030)
5X 930 dsDNA Inlet Buffer 125 mL (DNF-355-0125) 300 mL (DNF-355-0300)
5X Capillary Conditioning Solution 50 mL (DNF-475-0050) 100 mL (DNF-475-0100)
35 bp and 1,500 bp Markers 3.2 mL (FS-SMK910-0003) 3.2 mL (FS-SMK910-0003)
100 bp DNA Ladder 1.0 mL (FS-SLR910-0001) 1.0 mL x 2 (FS-SLR910-0001)
Mineral Oil 15 mL (FS-SMO15) 15 mL (FS-SMO15)
Dilution Buffer 1X TE 60 mL (DNF-495-0060) 125 mL (DNF-495-0125)

Specifications

Specifications Description
Sample Volume Required 2 µL
Diluent Marker Volume Required 22 µL
DNA Sizing Range 35 bp – 1,500 bp (defined by lower/upper marker)
Separation Resolution Ultra-Short Array: 35 bp – 100 bp ≤ 10%; 100 bp – 700 bp ≤ 5%; 700 - 1,500 bp ≤ 5%
Short Array: 35 bp – 100 bp ≤ 10%; 100 bp – 1,500 bp ≤ 5%
Long Array: 35 bp – 100 bp ≤ 10%; 100 bp – 1,500 bp ≤ 5%
DNA Sizing Accuracy ± 5% or better
DNA Sizing Precision 2% CV
DNA Fragment Concentration Range 0.5 ng/µL – 50 ng/µL input DNA
Total Electrophoresis Run Time 22 min (Ultra-Short Array); 45 min (Short Array); 70 min (Long Array)

Examples

Qualitative analysis of DNA requires a well separated, quality DNA ladder for sizing samples. The 100 bp DNA Ladder (FS-SLR910-0001) provides sizing from 35 bp – 1,500 via well separated ladder fragments and markers. The sizing capability provided by the ladder, shown in Figure 1, enables the analysis of multiple sample types, including microsatellites, single DNA fragments, and amplicons from multiplexed PCR.

Figure 1
Figure 1. A separation of the 100 bp DNA Ladder (FS-SLR910-0001) carried out on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the dsDNA 910 Reagent Kit (DNF-910).

Single DNA fragments, regardless of source, are readily electrophoresed and sized using the dsDNA 910 Reagent Kit (DNF-910). An example separation is depicted in Figure 2, the amplicon from a PCR of a viral sample.

Figure 2
Figure 2. A PCR amplicon from a viral sample separated using the dsDNA 910 Reagent Kit (DNF-910). Capillary electrophoresis was performed on a Fragment Analyzer equipped with a Short Capillary Array (33-55). Left Panel: Electropherogram of the separation. Right Panel: Digital gel image (Gaussian Fit, Non-integrated Peaks Hidden).

Electrophoresis of multiple DNA fragments in a single sample has often proven challenging for other methods due to poor separation resolution. Products from a multiplex PCR – 18 in total – with amplicons sized between 100 bp and 510 bp, were separated as shown in Figure 3.

Figure 3
Figure 3. Multiplex PCR amplicons separated using the dsDNA 910 Reagent Kit (DNF-910). Capillary electrophoresis was performed on a Fragment Analyzer equipped with a Short Capillary Array (33-55). Left Panel: Electropherogram of the 18 amplicon separation. Right Panel: Digital gel image of the 18 amplicon separation (Gaussian Fit, Non-Integrated Peaks Hidden).

The 18 fragments, sized as closely as 14 bp, were separated with distinct peaks formed for each amplicon. Such fine resolution contributes to the accurate (± 5%) and precise (2% CV) sizing of the kit. For those familiar with agarose electrophoresis, the exceptional separation resolution is perhaps best viewed via the digital gel image (Figure 3, Right Panel), bands are crisp and well separated despite the close sizes of many of the fragments.

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