Reagent Kits

AATI's capillary electrophoresis instruments utilize specially designed reagent kits to analyze diverse nucleic acid samples, from small RNA to genomic DNA, and NGS library quality control.

Reagent Kit Box

dsDNA 905 Reagent Kit

dsDNA 905 Reagent Kit
500 Sample dsDNA 905 Reagent Kit

The dsDNA 905 Reagent Kit is a qualitative kit for the automated and accurate sizing of DNA fragments between 1 bp and 500 bp. Specifically, this kit was designed for challenging samples; utilizing a specially designed gel to that enhances separation. Common applications of this kit include genotyping, the analysis of PCR amplicons, microsatellites, and SSRs. Note: This is a qualitative kit for the accurate sizing and relative quantification of fragments.

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Components

Components 500 Sample Kit 1,000 Sample Kit
dsDNA 905 Separation Gel 240 mL (DNF-905-0240) 500 mL (DNF-905-0500)
Intercalating Dye 30 µL (DNF-600-U030) 30 µL x 2 (DNF-600-U030)
5X 930 dsDNA Inlet Buffer 125 mL (DNF-355-0125) 300 mL (DNF-355-0300)
5X Capillary Conditioning Solution 50 mL (DNF-475-0050) 100 mL (DNF-475-0100)
1 bp and 500 bp Markers 3.2 mL (FA-MRK900F-0003) 3.2 mL (FA-MRK900F-0003)
35 bp – 400 bp Range DNA Ladder 1.0 mL (FS-SLR905-0001) 1.0 mL x 2 (FS-SLR905-0001)
Mineral Oil 15 mL (FS-SMO15) 15 mL (FS-SMO15)
Dilution Buffer 1X TE 60 mL (DNF-495-0060) 125 mL (DNF-495-0125)

Specifications

Specifications Description
Sample Volume Required 2 µL (adjustable depending upon sample concentration)
Diluent Marker Volume Required 22 µL
DNA Sizing Range 1 bp – 500 bp (defined by lower/upper marker)
Separation Resolution Ultra-Short Array: 8-10 bp @ 300 bp
Short Array: 5 bp @ 300 bp
Long Array: 3-5 bp @ 300 bp
DNA Sizing Accuracy ± 5% or better
DNA Sizing Precision 2% CV
DNA Fragment Concentration Range 0.5 ng/µL – 50 ng/µL input DNA
Total Electrophoresis Run Time 28 min (Ultra-Short Array, 22-77); 60 min (Short Array, 33-55); 80 min (Long Array, 55-80)

Examples

Qualitative analysis of DNA requires a well separated, quality, DNA ladder. The 35 bp – 400 bp Range DNA Ladder provides accurate sizing from 1 bp to 500 bp via well separated ladder fragments and markers. This kit is ideal for the analysis of PCR amplicons, microsatellites, and genotyping applications.

Figure 1
Figure 1. A typical separation of the 35 bp - 400 bp Range DNA Ladder (FS-SLR905) carried out on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the dsDNA 905 Reagent Kit (DNF-905).

The accurate sizing and relative quantification of DNA is a crucial aspect of many molecular biology workflows. Some DNA samples prove more challenging to size and quantify than others, interrupting workflow and prolonging acquisition of results. A variety of factors can complicate the electrophoresis of DNA, including nucleotide sequence composition and structural conformation. The dsDNA 905 Reagent Kit (DNF-905) was specially designed to ameliorate the issues associated with challenging samples such as size shifts and shadow peaks. Figure 2 depicts a 120 bp amplicon separated using the dsDNA 905 Reagent Kit (DNF-905).

The amplicon separated in Figure 2 had previously shown shadow peaks and size shifts. Upon separation with the specialized gel included in the dsDNA 905 Reagent Kit (DNF-905) the previous issues with the amplicon shown in Figure 2 were alleviated.

Figure 2
Figure 2. Separation of a single amplicon using the dsDNA 905 Reagent Kit (DNF-905) on a Fragment Analyzer equipped with a Short Capillary Array (33-55).

The dsDNA 905 Reagent Kit provides superior resolution, easily distinguishing between closely sized fragments. Figure 3 depicts 4 bp resolution between two amplicons, 119 bp and 123 bp respectively. Such fine resolution is made possible through the careful selection of ladder fragments and a high quality separation gel.

Figure 3
Figure 3. Overlay of two closely sized amplicons, 119 bp and 123 bp respectively. Separations performed using the dsDNA 905 Reagent Kit (DNF-905) on a Fragment Analyzer equipped with a Short Capillary Array (33-55).
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