Reagent Kits

AATI's capillary electrophoresis instruments utilize specially designed reagent kits to analyze diverse nucleic acid samples, from small RNA to genomic DNA, and NGS library quality control.

Reagent Kit Box

dsDNA 900 Reagent Kit

dsDNA 900 Reagent Kit
500 Sample dsDNA 900 Reagent Kit

The dsDNA 900 Reagent Kit is a qualitative kit for the automated and accurate sizing of DNA fragments between 35 bp and 500 bp. Common applications of this kit include genotyping, the analysis of PCR amplicons, microsatellites, and SSRs. Note: This is a qualitative kit for the accurate sizing and relative quantification of fragments.

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Components

Components500 Sample Kit1,000 Sample Kit
dsDNA 800 Separation Gel240 mL (DNF-800-0240)500 mL (DNF-800-0500)
Intercalating Dye30 µL (DNF-600-U030)30 µL x 2 (DNF-600-U030)
5X 930 dsDNA Inlet Buffer125 mL (DNF-355-0125)300 mL (DNF-355-0300)
5X Capillary Conditioning Solution50 mL (DNF-475-0050)100 mL (DNF-475-0100)
35 bp and 500 bp Markers3.2 mL (FS-SMK900-0003)3.2 mL (FS-SMK900-0003)
75 – 400 bp Range DNA Ladder1.0 mL (FS-SLR905-0001)1.0 mL x2 (FS-SLR905-0001)
Mineral Oil15 mL (FS-SMO15)15 mL (FS-SMO15)
Dilution Buffer 1X TE60 mL (DNF-495-0060)125 mL (DNF-495-0125)

Specifications

SpecificationsDescription
Sample Volume Required2 µL (adjustable depending upon sample concentration)
1X TE Volume Required22 µL
DNA Sizing Range35 bp – 500 bp (defined by lower/upper marker)
Separation ResolutionUltra-Short Array: 8-10 bp @ 300 bp;
Short Array: 5-10 bp @ 300 bp
Long Array: 3-5 bp @ 300 bp
DNA Sizing Accuracy± 5% or better
DNA Sizing Precision2% CV
DNA Fragment Concentration Range0.5 ng/µL – 50 ng/µL input DNA (adjustable by dilution of sample)
Total Electrophoresis Run Time28 min (Ultra-Short Array, 22-47); 60 min (Short Array, 33-55); 80 min (Long Array, 55-80)

Examples

Qualitative analysis of DNA requires a well separated, quality, DNA ladder. The 75 bp – 400 bp Range DNA Ladder provides accurate sizing from 35 bp – 500 bp via well separated ladder fragments and markers. This ladder is ideal for the analysis of PCR fragments, microsatellites, and genotyping applications.

Figure 1
Figure 1. A typical separation of the 75 bp - 400 bp Range DNA Ladder (FS-SLR900-0001) carried out on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the dsDNA 900 Reagent Kit (DNF-900).

Without regard to source, dsDNA 900 Reagent Kit (DNF-900) separates and sizes DNA fragments between 35 bp and 500 bp with unparalleled accuracy and precision (±5% and 2%, respectively). An example of a single fragment separation is shown in Figure 2.

Figure 2
Figure 2. A separation of a single amplicon using the dsDNA 900 Reagent Kit (DNF-900). Electrophoresis was performed on a Fragment Analyzer equipped with a Short Capillary Array (33-55). Left Panel: Electropherogram. Right Panel: Digital Gel Image (Gaussian Fit, Non-Integrated Peaks Hidden).

The electropherogram (left) and digital gel image (right) represent the same data, as such, if a sample provides a clear, distinct peak on the electropherogram a correspondingly clear band will appear on the digital gel image. This is shown as clearly with multiple fragment samples as in single fragment samples. Figure 3 depicts a separation of a sample with 4 amplicons: 140 bp, 146 bp, 152 bp and 159 bp.

Figure 3
Figure 3. A separation of multiple amplicons using the dsDNA 900 Reagent Kit (DNF-900). Electrophoresis was performed on a Fragment Analyzer equipped with a Short Capillary Array (33-55). Left Panel: Electropherogram. Right Panel, Digital Gel Image (Gaussian Fit, Non-Integrated Peaks Hidden).

The separation resolution is readily apparent in Figure 3, four DNA fragments between 140 bp to 160 bp were distinguished on both the electropherogram and the digital gel image. The dsDNA 900 Reagent Kit (DNF-900) was able to resolve and size fragments as close as 6 bp to each other. Such resolving power makes the dsDNA 900 Reagent Kit (DNF-900) ideal for the precision sizing of small DNA fragments of all sources.

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