Reagent Kits

Our capillary electrophoresis instruments utilize specially designed reagent kits to analyze diverse nucleic acid samples, from small RNA to genomic DNA, and NGS library quality control.

Reagent Kit Box

Large Fragment Kit

The Large Fragment Kit (DNF-492) is used for the automated sizing and quantification of large DNA fragments and smears. Common applications include as a quality assurance step in long read sequencing, checking the quality of a DNA extraction, and evaluating the quality of degraded DNA samples. Ideal for high concentration samples, this kit is able to size samples through 20 Kb.

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Components500 Sample Kit1,000 Sample Kit
Large Fragment Separationg Gel240 mL (DNF-220-0240)500 mL (DNF-220-0240)
Intercalating Dye30 µL (DNF-600-U030)30 µL x 2 (DNF-600-U030)
5X 930 dsDNA Inlet Buffer125 mL (DNF-355-0125)300 mL (DNF-355-0300)
5X Capillary Conditioning Solution50 mL (DNF-475-0050)100 mL (DNF-475-0100)
SS Large Fragment Diluent Marker Solution2.4 mL x 5 vials (DNF-380-0003)2.4 mL x 10 vials (DNF-380-0003)
SS Large Fragment DNA Ladder100 µL (DNF-378-U100)100 µL x 2 (DNF-378-U100)
0.6X TE Rinse Buffer125 mL (DNF-496-0125)125 mL (DNF-496-0125)
BF-2000 Blank Solution8 mL (DNF-302-0008)8 mL (DNF-302-0008)


Sample Volume Required2 µL
Diluent Marker Volume Required22 µL
DNA Sizing Range50 bp – 20,000 bp
DNA Sizing Accuracy± 15% or better
DNA Fragment Concentration Range0.1 ng/µL - 10 ng/µL input DNA
DNA Smear Concentration Range5 ng/µL - 100 ng/µL input DNA
DNA Quantification Accuracy± 25%
DNA Quantification Precision20% CV
Maximum DNA Concentration10 ng/µL per fragment; 100 ng/µL total
Total Electrophoresis Run Time 50 minutes (Short Array, 33-55); 80 minutes (Long Array, 55-80)


A typical separation of the Standard Sensitivity Large Fragment Ladder is depicted in Figure 1. A high quality ladder separation is crucial to the accurate quantification and sizing of large DNA fragments. The well separated ladder fragments allow for the accurate sizing of large DNA fragments through 20,000 bp and accurately quantifies fragments as low as 0.1 ng/µL.

Figure 1
Figure 1. A typical separation of the Standard Sensitivity Large Fragment DNA Ladder (DNF-378) on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the Large Fragment Kit (DNF-492).

Through the use of high quality reagents and carefully selected electrophoresis parameters, the Large Fragment Kit (DNF-492) is able to accurately measure large DNA fragment through 20 Kb. Figure 2 depicts the separation of a synthetic 15 Kb DNA fragment using this kit. In this example, ProSize measured this fragment to within 3% of its known size.

Figure 2
Figure 2. A synthetic 15 Kb DNA fragment separated on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the Large Fragment Kit (DNF-492).

Efficient and accurate sizing of large DNA fragments is a significant aspect of many molecular biology applications including next generation long read sequencing, assessment of purification success, and generation of large synthetic gene constructs, among others. Notably, assessment of large DNA fragments is crucial for Human Leukocyte Antigen (HLA) typing, a process essential for matching donor organ and tissue to recipients. Click here to learn more about how the Anthony Nolan Research Institute uses the large fragment kits for the Fragment Analyzer in HLA typing workflows.

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