Reagent Kits

AATI's capillary electrophoresis instruments utilize specially designed reagent kits to analyze diverse nucleic acid samples, from small RNA to genomic DNA, and NGS library quality control.

Reagent Kit Box

High Sensitivity Genomic DNA Analysis Kit

High Sensitivity Genomic DNA Analysis Kit
500 Sample High Sensitivity Genomic DNA Analysis Kit

The High Sensitivity Genomic DNA Analysis Kit is used for the automated analysis of low concentration genomic DNA in the 40 Kb range. Common applications include the evaluation of samples for next generation sequencing. It is also suited to the analysis of degraded DNA, such as FFPE preserved samples. Further streamlining workflows, the Genomic Quality Number (GQN) is calculated in PROSize® 2.0 post-separation, providing a user-defined metric of sample quality. Note: This kit only uses a lower marker.

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Components

Components 500 Sample Kit 1,000 Sample Kit
Genomic DNA Separation Gel 240 mL (DNF-270-0240) 500 mL (DNF-270-0500)
Intercalating Dye 30 µL (DNF-600-U030) 30 µL x 2 (DNF-600-U030)
5X 930 dsDNA Inlet Buffer 125 mL (DNF-355-0125) 300 mL (DNF-355-0300)
5X Capillary Conditioning Solution 50 mL (DNF-475-0050) 100 mL (DNF-475-0100)
HS Genomic DNA Diluent Marker Solution 2.4 mL x 5 vials (DNF-375-0003) 2.4 mL x 10 vials (DNF-375-0003)
HS Genomic DNA Ladder 100 µL (DNF-377-U100) 100 µL x 2 (DNF-377-U100)
0.25X TE Rinse Buffer 125 mL (DNF-497-0125) 125 mL (DNF-497-0125)
BF-25 Blank Solution 8 mL (DNF-300-0008) 8 mL (DNF-300-0008)

Specifications

Specifications Description
Sample Volume Required 2 µL
Diluent Marker Volume Required 22 µL
DNA Sizing Range 50 bp – 40,000 bp
gDNA Concentration Range 50 pg/µL - 5 ng/µL input gDNA
gDNA Quantification Accuracy ± 25%
gDNA Quantification Precision ± 15% CV
Maximum gDNA Concentration 5 ng/µL
Total Electrophoresis Run Time 30 min (Ultra-Short Array, 22-47); 50 min (Short Array, 33-55); 80 min (Long Array, 55-80)

Examples

A typical separation of the High Sensitivity Genomic DNA Ladder (DNF-377) is shown in Figure 1. Electrophoresis was carried out on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the High Sensitivity Genomic DNA Analysis Kit (DNF-488). The clearly defined ladder peaks allow for the accurate measurement of genomic DNA samples, with an extended sizing range through 40,000 bp. The High Sensitivity Genomic DNA Analysis Kit (DNF-488) provides numerous advantages, including saving precious sample along with accurate and precise sizing through 40 Kb.

Figure 1
Figure 1. A typical separation of the High Sensitivity Genomic DNA Ladder (DNF-377) performed on a Fragment Analyzer equipped with a Short Capillary Array (33-55) with the High Sensitivity Genomic DNA Analysis Kit (DNF-488).

A separation of human gDNA with a Genomic Quality Number (GQN) of 9.6 is shown in Figure 2. Preparations of gDNA have a high degree of variation in quality for numerous reasons – including purification technique, age, and sample source – to the point that a fixed quality metric is of limited use. The GQN allows users to define what good quality gDNA is for their distinctive applications. The GQN is measured as the amount of sample that exceeds a user-selected size threshold, the resulting measurement reported on a scale of 0 to 10 with higher values corresponding to more of the sample falling above the defined threshold. The GQN changes with the selected threshold, if the user defines a threshold of 20,000 bp instead of the 10,000 bp threshold used in Figure 2, the GQN will be lower. An adaptive metric like the GQN provides users with more versatility in their analysis of diverse samples, as different downstream applications and sample sources have differing degrees of what qualifies as "good" gDNA.

Figure 2
Figure 2. Human gDNA separation, performed on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the High Sensitivity Genomic DNA Analysis Kit (DNF-488). The dotted blue line depicts the user-selected threshold of 10,000 bp for the determination of GQN. The GQN for this sample with a user-defined threshold of 10,000 bp is 9.6.
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