Reagent Kits

AATI's capillary electrophoresis instruments utilize specially designed reagent kits to analyze diverse nucleic acid samples, from small RNA to genomic DNA, and NGS library quality control.

Reagent Kit Box

Standard Sensitivity NGS Fragment Analysis Kit

Standard Sensitivity NGS Fragment Analysis Kit
500 Standard Sensitivity NGS Fragment Analysis Kit

The Standard Sensitivity NGS Fragment Analysis Kit provides an automated solution to the quality assessment of high concentration NGS libraries prior to sequencing. The correct determination of molarity for optimum flow-cell loading is a critical step in NGS, and DNF-473 efficiently and accurately determines the average size of a library and its concentration. An accurate and precise molarity calculation helps ensure quality NGS results. Use DNF-473 to increase the efficiency of your genomic research.

Back to Kit List

Components

Components500 Sample Kit1,000 Sample Kit
NGS Fragment Separation Gel240 mL (DNF-240-0240)500 mL (DNF-240-0500)
Intercalating Dye30 µL (DNF-600-U030)30 µL x 2 (DNF-600-U030)
5X 930 dsDNA Inlet Buffer125 mL (DNF-355-0125)300 mL (DNF-355-0300)
5X Capillary Conditioning Solution50 mL (DNF-475-0050)100 mL (DNF-475-0100)
SS NGS Fragment Diluent Marker Solution2.4 mL x 5 vials (DNF-374-0003)2.4 mL x 10 vials (DNF-374-0003)
SS NGS Fragment DNA Ladder100 µL (DNF-399-U100)100 µL x 2 (DNF-399-U100)
0.6X TE Rinse Buffer125 mL (DNF-496-0125)125 mL (DNF-496-0125)
BF-2000 Blank Solution8 mL (DNF-302-0008)8 mL (DNF-302-0008)

Specifications

SpecificationsDescription
Sample Volume Required2 µL
Diluent Marker Volume Required22 µL
DNA Sizing Range100 bp – 6,000 bp
Separation ResolutionShort Array: 25 bp - 100 bp ≤ 10%; 100 bp - 2000 bp ≤ 5%; 2000 bp - 5000 bp ≤ 10%
DNA Sizing Accuracy± 5% or better
DNA Sizing Precision2% CV
DNA Fragment Concentration Range0.1 ng/µL - 10 ng/µL input DNA
DNA Smear Concentration Range5 ng/µL - 100 ng/µL input DNA
DNA Quantification Accuracy± 25 %
DNA Quantification Precision15 % CV
Maximum DNA Concentration10 ng/µL per fragment; 100 ng/µL total
Total Electrophoresis Run Time50 minutes (Short Array, 33-55); 80 minutes (Long Array, 55-80)

Examples

Figure 1 depicts a typical separation of the Standard Sensitivity NGS DNA Ladder (DNF-374). The peaks are well separated and clearly defined, providing a standard curve for the sizing and quantification of DNA smears, namely NGS libraries.

Figure 1
Figure 1. A typical separation of the Standard Sensitivity NGS DNA Ladder (DNF-374) performed on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the Standard Sensitivity NGS Fragment Analysis Kit.

An example of a high quality NGS library is depicted in Figure 2. Signs of a successful library preparation include:

  • A smooth, defined smear of the desired average size
  • No Primer Dimers
  • No Adapter Dimers

Conversely, a poor quality separation is stipulated by the lack of one or more of the characteristics of a high quality NGS smear. An NGS library of poor quality is more likely to compromise sequencing results, decreasing depth and providing lower quality reads.

Figure 2
Figure 2. High quality NGS library separated on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the Standard Sensitivity NGS Fragment Analysis Kit (DNF-473).
Top