Reagent Kits

AATI's capillary electrophoresis instruments utilize specially designed reagent kits to analyze diverse nucleic acid samples, from small RNA to genomic DNA, and NGS library quality control.

Reagent Kit Box

Standard Sensitivity NGS Fragment Analysis Kit

Standard Sensitivity NGS Fragment Analysis Kit
500 Standard Sensitivity NGS Fragment Analysis Kit

The Standard Sensitivity NGS Fragment Analysis Kit provides an automated solution to the quality assessment of high concentration NGS libraries prior to sequencing. The correct determination of molarity for optimum flow-cell loading is a critical step in NGS, and DNF-473 efficiently and accurately determines the average size of a library and its concentration. An accurate and precise molarity calculation helps ensure quality NGS results. Use DNF-473 to increase the efficiency of your genomic research.

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Components

Components 500 Sample Kit 1,000 Sample Kit
NGS Fragment Separation Gel 240 mL (DNF-240-0240) 500 mL (DNF-240-0500)
Intercalating Dye 30 µL (DNF-600-U030) 30 µL x 2 (DNF-600-U030)
5X 930 dsDNA Inlet Buffer 125 mL (DNF-355-0125) 300 mL (DNF-355-0300)
5X Capillary Conditioning Solution 50 mL (DNF-475-0050) 100 mL (DNF-475-0100)
SS NGS Fragment Diluent Marker Solution 2.4 mL x 5 vials (DNF-374-0003) 2.4 mL x 10 vials (DNF-374-0003)
SS NGS Fragment DNA Ladder 100 µL (DNF-399-U100) 100 µL x 2 (DNF-399-U100)
0.6X TE Rinse Buffer 125 mL (DNF-496-0125) 125 mL (DNF-496-0125)
BF-2000 Blank Solution 8 mL (DNF-302-0008) 8 mL (DNF-302-0008)

Specifications

Specifications Description
Sample Volume Required 2 µL
Diluent Marker Volume Required 22 µL
DNA Sizing Range 100 bp – 6,000 bp
Separation Resolution Short Array: 25 bp - 100 bp ≤ 10%; 100 bp - 2000 bp ≤ 5%; 2000 bp - 5000 bp ≤ 10%
DNA Sizing Accuracy ± 5% or better
DNA Sizing Precision 2% CV
DNA Fragment Concentration Range 0.1 ng/µL - 10 ng/µL input DNA
DNA Smear Concentration Range 5 ng/µL - 100 ng/µL input DNA
DNA Quantification Accuracy ± 25 %
DNA Quantification Precision 15 % CV
Maximum DNA Concentration 10 ng/µL per fragment; 100 ng/µL total
Total Electrophoresis Run Time 50 minutes (Short Array, 33-55); 80 minutes (Long Array, 55-80)

Examples

Figure 1 depicts a typical separation of the Standard Sensitivity NGS DNA Ladder (DNF-374). The peaks are well separated and clearly defined, providing a standard curve for the sizing and quantification of DNA smears, namely NGS libraries.

Figure 1
Figure 1. A typical separation of the Standard Sensitivity NGS DNA Ladder (DNF-374) performed on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the Standard Sensitivity NGS Fragment Analysis Kit.

An example of a high quality NGS library is depicted in Figure 2. Signs of a successful library preparation include:

  • A smooth, defined smear of the desired average size
  • No Primer Dimers
  • No Adapter Dimers

Conversely, a poor quality separation is stipulated by the lack of one or more of the characteristics of a high quality NGS smear. An NGS library of poor quality is more likely to compromise sequencing results, decreasing depth and providing lower quality reads.

Figure 2
Figure 2. High quality NGS library separated on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the Standard Sensitivity NGS Fragment Analysis Kit (DNF-473).
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