The Small RNA Analysis Kit provides automated analysis of small RNAs. The kit excels in the evaluation of small RNAs for downstream applications, notably including small RNA NGS libraries. Able to distinguish between precursor small RNA and functional small RNA, it can also quantify the amount of microRNA present in any given sample. To help streamline laboratory workflows, post-separation analysis using the Small RNA Analysis Region function in PROSize® 2.0 easily quantifies the microRNA and small RNA present in a sample. Note: This kit is optimized for use on a 12 capillary array.
|275 Sample Kit|
|Small RNA Separation Gel||250 mL (DNF-262-0250)|
|Intercalating Dye||30 µL (DNF-600-U030)|
|5X 930 dsDNA Inlet Buffer||125 mL (DNF-355-0125)|
|5X Capillary Conditioning Solution||50 mL (DNF-475-0050)|
|Small RNA Diluent Marker||4 mL x 2 (DNF-368-0004)|
|Small RNA Ladder||60 µL (DNF-361-U060)|
|0.25X TE Rinse Buffer||125 mL (DNF-497-0125)|
|BF-25 Blank Solution||8 mL (DNF-300-0008)|
|Eppendorf LoBind® 0.5 mL tubes||package of 50|
|Sample Volume Required||2 µL|
|Diluent Marker Volume Required||18 µL|
|RNA Sizing Range||5 - 200 nt|
|Qualitative Range||25 pg/µL - 2500 pg/µL (microRNA region)|
|Quantitative Range||50 pg/µL - 2000 pg/µL (microRNA region)|
|Quantification Precision||25% CV (Small RNA Ladder)|
|Total Electrophoresis Run Time||17 minutes (Ultra-Short Array, 22-47); 22 minutes (Short Array,33-55)|
With the increasing interest in small RNAs for downstream applications such as NGS, the accurate and efficient analysis of small RNAs from a variety of sources is crucial. Small RNA sample analysis is dependent upon the employment of a high quality small RNA ladder as seen in Figure 1. The Small RNA Analysis Kit (DNF-470) is able to measure the size and concentration of small RNAs, including functional microRNAs, due to the Small RNA Ladder (DNF-361). The Small RNA Ladder, comprised of small RNA fragments, enhances the accuracy and precision of fragment size and concentration measurements beyond other analysis methods.
The Small RNA Analysis Kit (DNF-470) provides a more detailed analysis of the small RNA region than possible with the HS Total RNA Analysis Kit (DNF-472) as shown in Figure 2. The same total RNA sample from Corn Leaf was run on the Fragment Analyzer using each respective analysis kit. The HS Total RNA Analysis Kit (DNF-472) provides an excellent overview of the sample, indicating that the extracted total RNA is high quality with an RQN of 10.0. The Small RNA Analysis Kit (DNF-470) takes the analysis a step further with not only a more detailed separation of the small RNA region, but quantifying the amount of small RNA and microRNA present in the total RNA sample.