Reagent Kits

Our capillary electrophoresis instruments utilize specially designed reagent kits to analyze diverse nucleic acid samples, from small RNA to genomic DNA, and NGS library quality control.

Reagent Kit Box

HS Genomic DNA 50kb Kit

The HS Genomic DNA 50kb Kit (DNF-468) is ideal for the automated quality assessment of gDNA for downstream applications such as next generation sequencing. A broad sizing range allows researchers to accurately size gDNA samples through 60 Kb. Perfect for precious or low concentration samples, use only 2 µL of sample and quantify samples with concentrations as low as 0.3 ng/µL.

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Components

500 Sample Kit
Genomic DNA Separation Gel240 mL (DNF-270-0240)
Intercalating Dye30 µL (DNF-600-U030)
5X 930 dsDNA Inlet Buffer125 mL (DNF-355-0125)
5X Capillary Conditioning Solution50 mL (DNF-475-0050)
HS Genomic DNA Diluent Marker Solution2.4 mL x 5 vials (DNF-375-0003)
High Sensitivity Extended Genomic DNA Ladder125 µL (DNF-364-U125)
0.25X TE Rinse Buffer125 mL (DNF-497-0125)
BF-25 Blank Solution8 mL (DNF-300-0008)
Eppendorf LoBind 0.5 mL TubesPackage of 50

Specifications

SpecificationsDescription
Sample Volume Required2 µL
Diluent Marker Volume Required22 µL
DNA Sizing Range75 bp – 60,000 bp
gDNA Concentration Range0.3 ng/µL - 12 ng/µL input gDNA
gDNA Quantification Accuracy± 30%
gDNA Quantification Precision25% CV
Maximum gDNA Concentration12 ng/µL
Total Electrophoresis Run Time 50 minutes (Ultrashort Array, 22-47); 60 minutes (33-55 Array)

Examples

The accurate sizing and quantification of genomic DNA necessitates the use of a high-quality DNA ladder. The HS Genomic DNA 50kb Kit employs the High Sensitivity Extended Genomic DNA Ladder (Figure 1) to reliably size and quantify gDNA samples. Sizing through 60 Kb is achieved through the inclusion of a 48.5 Kb DNA fragment. Reliable and accurate quantification of genomic DNA is achieved using carefully calibrated ladder fragment concentrations.

Figure 1
Figure 1. High Sensitivity Extended Genomic DNA Ladder (DNF-364) separated on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the HS Genomic DNA 50kb Kit (DNF-468).

Pure, high-quality genomic DNA is necessary for many applications from PCR to NGS. The quality assessment of genomic DNA is often dependent on subjective criteria and the interpretation of the researcher. Post-separation analysis in PROSize® Data Analysis Software alleviates these issues with clear depictions of separations in two formats – digital gel image and electropherogram (Figure 2) – and the use of a dynamic quality metric, the Genomic Quality Number (GQN). Specially designed for the changing demands of genomic DNA QC analysis, the GQN employs a user-defined size threshold. The GQN is reported as a value between 0 and 10, with high values corresponding to more of the gDNA sample falling above the user-selected size threshold. An example of the GQN is shown in Figure 2. In the example, a size threshold of 10,000 bp (shown as a dotted blue line) was selected, producing a GQN of 9.0.

Figure 1
Figure 2. Separation of high quality genomic DNA. Capillary electrophoresis was performed with a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the HS Genomic DNA 50kb Kit (DNF-468). The Peak Table, shown inset into the electropherogram, provides crucial information including: concentration, size threshold, and GQN.

For many applications, it is crucial to know the concentration of genomic DNA. The HS Genomic DNA 50kb Kit provides confident assessments of sample concentration. When compared to a fluorometric method of quantification, the Fragment Analyzer using the HS Genomic DNA 50kb Kit provides the same concentration values (Figure 3).

Figure 3a
Figure 3. A set of genomic DNA dilutions were quantified using a standard fluorometric instrument and the Fragment Analyzer with the HS Genomic DNA 50kb Kit (DNF-468). The obtained concentrations were plotted against each other, revealing a near one-to-one relationship between the methods.
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