Reagent Kits

AATI's capillary electrophoresis instruments utilize specially designed reagent kits to analyze diverse nucleic acid samples, from small RNA to genomic DNA, and NGS library quality control.

Reagent Kit Box
Order AccuCleave Agarose Kits

DNF-440-1000CP AccuCleaveTM T7CE

AccuCleave Agarose Kits
1000 Sample AccuCleave T7CE Kit

Streamline your CRISPR workflow with the AccuCleave T7CE Kit. This kit uses a rigorously tested heteroduplex assay to quickly identify gene editing events. Paired with the Fragment AnalyzerTM Automated CE System, the AccuCleave T7CE Kit provides a high throughput screening solution with unparalleled precision and accuracy.

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Components

Components 1000 Sample Kit
T7 Endonuclease I 8 µL (CP-END-U008)
10X Cleavage Buffer 2.2 mL (CP-CVB-0002)
Dilution Buffer 0.1X 200 mL (DNF-494-0200)
WT Control 20 µL (CP-PC1-U020)
-2 bp Control 20 µL (CP-PC5-U020)
Primer Set 1 20 µL (CP-PRM1-U020)

Examples

An accurate, high-throughput enzymatic screening method greatly enhances the efficiency of CRISPR workflows, minimizing the time and cost of NGS screening. The heteroduplex assay in the AccuCleave T7CE Kit quickly and efficiently identifies positive clones and approximates zygosity. Visualization of these results is carried out with the CRISPR Gel Discovery Kit (DNF-910-1000CP) on the Fragment Analyzer™ Automated CE System, shown in Figure 1.

Figure 1
Figure 1. Visualization of AccuCleave T7CE assay using the CRISPR Gel Discovery Kit (DNF-910-1000CP) on the Fragment Analyzer™ Automated CE System. Data analysis performed using PROSize® Data Analysis Software to determine percent cleaved and zygosity.

Analysis in PROSize® Data Analysis Software enables the determination of percent cleaved and approximation of zygosity. In a 2n system, CRISPR mutations have three potential zygosities:

  • Monoallelic: mutation present in only one allele; genotype mutant1/WT
  • Diallelic Homozygous: identical mutation on both alleles; genotype mutant1/mutant1
  • Diallelic Heterozygous: distinct mutations on each allele; genotype mutant1/mutant2

Further downstream analysis with NGS or Sanger Sequencing is necessary to characterize the exact nature of the gene editing events.

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