AccuCleave Agarose

Developed for low-throughput, agarose-based laboratories, AccuCleave – Agarose provides uncompromising detection of CRISPR/Cas9 gene editing events.

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DNF-441-0015CP

DNF-441-0015CP AccuCleaveTM C15 Control Kit

AccuCleave Agarose Kits
100 Sample AccuCleave C15 Control Kit

The AccuCleave C15 Control Kit provides researchers with DNA controls for the AccuCleave family of enzyme cleavage kits. The kit includes 15 DNA controls providing users with rigorously designed reaction controls for heteroduplex cleavage assays.

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Components

Components 100 Sample Kit
WT Control 20 µL (CP-PC1-U020)
+1 bp Control 20 µL (CP-PC2-U020)
-1 bp Control 20 µL (CP-PC3-U020)
+2 bp Control 20 µL (CP-PC4-U020)
-2 bp Control 20 µL (CP-PC5-U020)
+10 bp Control 20 µL (CP-PC6-U020)
-10 bp Control 20 µL (CP-PC7-U020)
G-T Control 20 µL (CP-PC8-U020)
G-G Control 20 µL (CP-PC9-U020)
T-T Control 20 µL (CP-PC10-U020)
A-A Control 20 µL (CP-PC11-U020)
A-G Control 20 µL (CP-PC12-U020)
C-C Control 20 µL (CP-PC13-U020)
T-C Control 20 µL (CP-PC14-U020)
A-C Control 20 µL (CP-PC15-U020)
Primer Set 1 50 µL (CP-PRM1-U050)

Examples

The AccuCleave C15 Control Kit includes 15 DNA controls: WT control, six InDel controls, and eight point mutations. Employed with the AccuCleave T7 (DNA-100-0100) or AccuCleave T7CE (DNF-440-1000CP) kits, it simultaneously supports assay optimization and provides simple confirmation of heteroduplex cleavage assay success. Figure 1 depicts separations of the WT Control, -1 bp Control, -2 bp Control, -10 bp Control, and the G-G Control.

Figure 1
Figure 1. WT Control, -1 bp Control, -2 bp Control, -10 bp Control, and the G-G Control separated using the CRISPR Gel Discovery Kit (DNF-910-1000CP) on a Fragment Analyzer equipped with a Short Capillary Array (33-55).

Additional Resources

  • DNA-441-0015CP AccuCleave C15 Control Kit User Manual
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