AATI’s Mutation Screening Method for CRISPR Workflows Featured in GEN
Published: Tuesday, November 28th, 2017
Tags: CRISPR, Fragment Analyzer
Integration of an accurate and efficient mutation screening method into CRISPR/Cas genome editing workflows saves researchers time and money by eliminating unnecessary sequencing.
Genetic Engineering & Biotechnology News recently published a tutorial detailing our solution for CRISPR mutation screening and zygosity determination. Starting with the AccuCleave T7CE Kit, cleavage products are separated using the CRISPR Gel Discovery Kit on the Fragment Analyzer, followed by analysis using a custom plugin for PROSize Data Analysis Software. For more information, read the tutorial here.
To learn more about all our CRISPR workflow solutions, including reagent QC, click here.