FASTEST Total Viable Organisms (TVO) Kit

Product Number:

• RBD-3000-50

Number of Tests:

• 800

Contents:

• Nucleic Acid (NA) Dye* Concentrate (4 x 40 µL)
• Nucleic Acid Dye Diluent (4 x 25 mL)
• BRAG3** (4 x 25 mL)
• Buffer Reagent (4 x 25 mL)
• 50 mL screw-cap conical tubes (4 x 25 mL)

*The viability stains in this product are sold under license from Molecular Probes, Inc., for use in conjunction with Rapid Bacterial Detector instruments.
**BRAG3 is a patented solution.

 

How the FASTEST TVO Kit Works

A 3 mL aliquot of pH-neutral sample is needed for analysis (buffer may be automatically added per the appropriate method to adjust the stock sample to a neutral pH of 6-8). Various dyes are used in the analysis: Nucleic Acid Dye is a membrane-permeable dye that stains live and dead cells. Using NA Dye alone gives information on the total number of live and dead cells in the sample. BRAG3 is a non-membrane permeable counter-stain that decreases the contribution of background counts due to extraneous debris, including dead cells. BRAG3 is a patented solution. Samples are analyzed on the Micro PRO™ using a defined method and a direct-read, counts/ml format.

Diagram of the FASTEST Total Viable Organisms Staining Protocol

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