Home > AdvanCE™ FS96 > PCR Fragment Analysis Examples
PCR Fragment Analysis Examples
The AdvanCE™ FS96 dsDNA Fluorescent System can be used to separate and analyze nucleic acid fragments from any type of PCR reactions including microsatellites, STR, SSR, VNTR, SNP, etc. It can also be used to evaluate larger fragments of DNA from restriction digestions as well as plasmid DNA
PCR fragment analysis
Example #1 - PCR fragment analysis.
Separation of amplicon (300, 500 and 700 bp fragments). 100bp ladder is shown on right side.
Example #2 - PCR fragment analysis.
Separation of amplicon (300 and 425 bp fragments). LMW ladder is shown on right and left side.
Example #3 - Large fragment analysis.
Separation of digested plasmid DNA. 1kb ladder is shown on the left side.
Gels information
| Part No. | Usage |
|---|---|
| DNF-900-0450 | Best for small fragment analysis, 10-500 bp, resolution approximately 2 bp |
| DNF-910-0450 | Broad range gel, analyze fragments 50 – 2,000 bp. Resolution over range: 5bp <500bp fragments; 5-10bp, 500-1,000bp fragments; 50-100bp,> 1,000 |
| DNF-920-0450 | Large and medium size fragment analysis, 500 – 40,000bp. Gel is also capable of separating plasmid DNA, supercoiled, relaxed and linear species. |
