Application

Chiral Analysis

Chiral analysis is an essential undertaking throughout Drug Discovery and Development since the enantiomeric forms of a chiral drug substance often possess completely different pharmacological effects — with one enantiomer producing a potent, desired effect and the other being inactive or even toxic.

Traditional methods for analytical-scale separation of enantiomers employ expensive, specialized chromatographic stationary phases that allow relatively low sample throughput. Advanced Analytical provides a tool enabling parallel chiral separations with a universal and tunable capillary array, resulting in significantly enhanced sample throughput while minimizing reagent costs and development times.

Key Benefits:

• The use of capillary electrophoresis provides a universal separation platform. Separation selectivity can be varied by simply exchanging buffer additives as opposed to changing column stationary phase chemistries.
• Low capillary volumes reduce reagent and sample consumption and lower the cost of analysis.
• The parallel system format allows for the screening of different sample/additive combinations in a single experiment, dramatically reducing method development cost and time.
• A specialized cooling system, combined with 50 µm i.d. capillaries, permit the use of either neutral or charged cyclodextrin chiral buffer additives.
• The system is flexible and can be readily configured for performing pKa measurements, log P measurements of neutral and basic compounds or analytical scale small molecule separations.

The above results show the parallel screening of (+/-) nefopam enantiomers against various sulfated cyclodextrin (CD) chiral selectors. The first peak in each capillary corresponds to 1,3,6,8-pyrenetetrasulfonate internal standard. The average enantiomeric resolution was: HS-α-CD, 2.86; HS-β-CD, 1.29; HS-γ-CD, 1.38; S-β-CD, 1.50.

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