I want to...

Stop Using Manual Slab Gels	Decrease Labor Prep Time
Improve Quantity/Quality Assessment	Increase Throughput

I want to Improve Quantity/Quality Assessment

Accurately assessing both the quality and quantity of plasmid DNA can be challenging. Until now, assessing quality (size) of plasmid DNA was typically done with agarose gel electrophoresis. Agarose gel, though easy to do, is inherently fraught with inconsistencies that can result from gel preparation variations and sample loading.

Also, ‘smiling effects’ caused by improper and uncontrolled electrophoresis conditions can lead to incorrect size assessment.Quantity analysis is equally tricky because UV and fluorometric methods look at all nucleic acids in the sample – not just the fragments of interest. Sometimes agarose gel is used for quantification. However, agarose can only provide ‘ballpark’ assessments, being subject to a myriad of experimental conditions.

The AdvanCE™ FS Nucleic Acids Analyzer improves both quality and quantity analysis of plasmid DNA for laboratories. Why? For starters, a specially formulated gel was developed to handle plasmid DNA separations.And by precisely controlling the electrophoresis conditions and separating the DNA through QC-performance tested gels and buffers, high separation resolutionsare achieved. The result is accurate sizing and quantification of linearized plasmid. Fragments are objectively quantified using analytical software specially designed and validated for this purpose.

The following factors allow the AdvanCE™ FS Nucleic Acids Analyzer to improve quality and quantity assessments of plasmid DNA:

1. Superior resolution of fragments.
2. Precise control of electrophoresis conditions and utilization of marker fragments.
3. PROSize™ analytical software.