I want to...

Automate Throughput	Decrease Labor Prep Time
Reduce Input gDNA	Stop Using Manual Slab Gels
Analzye Larger Fragments	Eliminate Clean Up Step
Increase Throughput	Reduce Time To Results
Eliminate Labeled Primers

I want to Reduce Input gDNA

In most experiments, a finite amount of genomic DNA is extracted from each leaf plug. Once this is used up, no more mutation detection analyses are feasible on these plants without either re-extraction of a frozen, stored leaf plug, or re-growing a plant from a stored M3 seed and then extracting DNA. Although that's possible, neither of these options are attractive since more work and time are required.

A better option would be to use less input gDNA in each PCR.

With the Mutation Detection Kit and the AdvanCE™ FS Nucleic Acids Analyzer, smaller PCR volumes can be used, saving precious gDNA and allowing more experiments to be run on each extraction. Using the current protocol for mutation detection only 2µL of the PCR product is required for analysis. An added advantage of smaller PCR volumes includes a requirement for less Taq, thus providing additional cost savings, or the ability to do more testing without additional PCR costs.

The following factors help reduce input genomic DNA for mutation detection on the AdvanCE™ FS Nucleic Acids Analyzer:

1. Small reaction volumes for digestion of heteroduplex fragments.
2. Efficient enzyme digestion.
3. High detection sensitivity of AdvanCE™ FS Nucleic Acids Analyzer.