I want to...

Automate Throughput	Decrease Labor Prep Time
Reduce Input gDNA	Stop Using Manual Slab Gels
Analzye Larger Fragments	Eliminate Clean Up Step
Increase Throughput	Reduce Time To Results
Eliminate Labeled Primers

I want to Automate Throughput

The conventional method for detecting unknown naturally-occurring and chemically-induced point mutations involves polyacrylamide gel electrophoresis and a labor-heavy intensive molecular method that's not designed for automation.

The older conventional method requires hours of hands-on time to:

• clean and assemble specialized glass plates.
• prepare unpolymerized gel and polymerization agents.
• pour the gel.
• set combs.
• prepare buffer for electrophoresis.

And this process MUST be repeated for every electrophoresis. Additionally, prior to the electrophoresis, the samples must be denatured at temperature after formamide addition then manually loaded onto the gels. In total, the process takes up to 9 hours for one experiment and over 2 hours of hands on time per 96 samples (see below).

With the AdvanCE™ FS Nucleic Acids Analyzer, most of these steps can be drastically reduced or eliminated altogether, as automation paves the way toward a streamlined workflow. The process employed with the AdvanCE™ FS Nucleic Acids Analyzer automates the analysis and essentially doubles daily throughput with a concomitant reduction in hands on time labor (see below).

The following design features help improve workflow and automation of mutation detection on the AdvanCE™ FS Nucleic Acids Analyzer:

1. Mutation Detection Kit (DNF-480-3000) and process that simplifies sample handling.
2. No gel or glass preparation requirements.
3. Elimination of Sephadex or ethanol precipitation clean up steps.