I want to...

Improve Separation Resolution	Improve Throughput
Increase Detection Sensitivity	Eliminate Use Of Labeled Primers
Stop Using Manual Slab Gels	Decrease Labor Prep Time
Shorten Electrophoresis Run Times	Lower Run Costs
Improve Quantity/Quality Assessment	Reduce Time to Results

I want to Eliminate Use Of Labeled Primers

Fluorescent labeled primers are more expensive than unlabelled primers. This fact dramatically increases the cost per unit to use fluorescent labeled primers in a PCR amplification. Other disadvantages to using fluorescent-labled primers are:

• their short half-life, some as brief as six months.
• Some primers are highly light sensitive.
• Some primers require special handling requirements.

Eliminating fluorescent-labeled primers has many advantages, the most obvious one being reduced primer costs. Yet another benefit - one that is often overlooked - is the ability to move directly from primer development to primer usage without amplification inconsistencies. And that means getting the research done faster.

Labeled primers are NOT needed on the AdvanCE™ FS Nucleic Acids Analyzer. We repeat: OUR SYSTEM DOES NOT REQUIRE THE USE OF FLUORESCENT-LABELED PRIMERS. Why is this so? Again, it gets back to the fact that we rely on an intercalating dye in the gel itself as our fluorescent source.

Eliminating the use of labeled primers can drop primer costs by a factor of five or more, depending on the label and level of purification needed after primer synthesis. Following design features of the AdvanCE™ FS Nucleic Acids Analyzer allow the elimination of labeled primers:

1. Use of nondenaturing gels and a sensitive intercalating dye.
2. A high wattage (1,000mW) LED and a sensitive CCD detector
3. Low signal to noise ratio, typically at 3:1