AdvanCE™ FS

I want to...

Automate Throughput Lower Run Cost
Decrease Labor Prep Time Increase Throughput
Improve Quantity/Quality Assessment Increase Detection Sensitivity

I want to Improve Quantity/Quality Assessment

All Next Gen Sequencing library preparations require quantity and quality analysis; for some instruments several analyses are needed. Critical to ensuring the optimum read length is accurate sizing and quantification of these fragments.

Each of the leading Next Gen Sequencing manufacturers typically “read” fragment lengths of varying sizes:

  • Applied Bio SOLiD™ — average fragment length of 50 base pairs.
  • Illumina GA - average fragment length of 75 to 100 base pairs.
  • Roche 454™ - average fragment length of 350 base pairs.
  • PacBio – average fragment length of 1,000 to 1,200 base pairs, while going as high as 6,000 bp.

Our technology achieves high resolution for fragment lengths of <500 bp - including two-bp for fragments <300 bp - and is the only CE instrument on the market capable of resolving fragments as large as 6,000 bp.

The AdvanCE™ FS Nucleic Acids Analyzer has been shown to have both better sizing and quantification accuracy/reproducibility than any other analytical methods (see Table). This means that results can be appraised with confidence for one sample, 96 samples and from one run to the next.

Specification Description
Sample Volume Required 2 µL
Number of Samples per Run 94 (+ 2 wells DNA Ladder) or 96 (Imported DNA Ladder)
Total Run Time 2 h (Including Conditioning and CE Separation)
DNA Sizing Range 50 bp - 5,000 bp
Separation Resolution 50-100bp: 5-10bp
100 bp - 2000bp < 5%
2000bp - 5000bp < 10%
DNA Sizing Accuracy ± 5% or better
DNA Sizing Precision 2% CV
DNA Fragment Concentration Range 5 pg/µL - 1000 pg/µL input DNA Concentration
DNA Smear Concentration Range 50 pg/µL - 5000 pg/µL input DNA Concentration
DNA Quantification Accuracy ± 25%
DNA Quantification Precision 15% CV
Maximum DNA Concentration 1000 pg/µL fragment; 5000 pg/µL smear

The following factors allow the AdvanCE™ FS Nucleic Acids Analyzer to improve quantity and quality analysis of NGS fragments:

  1. A specifically formulated QC performance tested Kit for NGS fragment analysis.
  2. Superior instrument design - coupling a sensitive detector with high quality optics system.
  3. Superior capillary design – separation over 80cm capillary versus <1 cm micro channel.