Monitor Microbial Fermentation Viability, Biomass

Microbial fermentation is an important component of industrial microbiology.  In the animal health, biopharmaceutical, pharmaceutical, nutraceutical, cosmeceutical, probiotic and biofuel industries, microbial fermentation is utilized for the manufacture of products such as drugs, enzymes, vaccines and nutraceuticals, as well as human and animal food additives.  The microbial fermentation industry holds significant interest in real-time viability monitoring, which allows for optimal harvest times and increased product yield.  The Micro PRO™ Detection System is a useful tool for determining biomass, viable and dead cell counts throughout all phases of the fermentation process.

Key Benefits:

• The Micro PRO™ provides reliable and quantitative results in 4 minutes.
• Micro PRO™ testing can be applied at various phases throughout the fermentation process, including:
• Upstream –detect media contamination, determine starter culture titer
• Fermentation –monitor biomass,  viable cells and/or dead cells
• Downstream –determine cell concentrations in in-process and in final product samples (e.g., inactivated bulks and concentrates)
• The system accurately enumerates a broad range of microorganisms, including fastidious organisms, mycoplasmas, gram positive and gram negative bacteria, yeasts, molds, spirochetes and anaerobes.
• Reagent kits are available for enumeration of biomass, viable organisms and dead cells.
• The Micro PRO™ is fully automated to performs reagent additions, mixing, and sample injection.
• The system holds a maximum of 42 samples, but also allows for continuous operation utilizing a batch process.
• The Micro PRO™ counts/mL correlate exceptionally well with traditional counting methods.
• Technical and validation support services are available.

Below are representative results that may be applicable to various industries:

• Industrial Microbiology – General
• Animal Health
• Probiotics
• Biofuels

Industrial Microbiology

Figure 1A.  Industrial Microbiology: Correlation of Micro PRO™ Viable Counts vs. Plate Counts of Escherichia coli (ATCC 8739) (each data point represents an average of 10 reps).

Figure 1B.  Industrial Microbiology: Reproducibility of Micro PRO™ Viable Counts vs. Plate Counts of Escherichia coli (ATCC 8739).

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Animal Health

Figure 2.  Animal Health: Correlation of Micro PRO™ Viable Counts vs Dilution of Mycoplasma hyopneumoniae (n=7).

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Probiotics

Figure 3.  Probiotics: Correlation of Micro PRO™ Viable Counts vs. Plate Counts of Bacillus subtilis (n=8).

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Biofuels

Figure 4A.  Biofuels: Correlation of Micro PRO™ Viable Counts vs. Plate Counts of Saccharomyces cerevisiae.

Figure 4B.  Biofuels: Reproducibility of Micro PRO™ Viable Counts vs. Plate Counts of Saccharomyces cerevisiae.

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