Synthetic Oligonucleotide Purity Analysis
Synthetic DNA and RNA oligonucleotides (oligos) are widely utilized as tools in disease diagnostics, medical and pharmaceutical research, and molecular biology. All of the DNA amplification methods including PCR use synthetic oligonucleotides (PCR primers). Accurate characterization of the purity of synthesized oligonucleotides is imperative, as impurities can significantly affect performance, reproducibility and overall success of DNA- and RNA-based application methods.
The Oligo PRO™ system from Advanced Analytical provides a rapid, accurate and economical method for performing high resolution, size-based synthetic oligonucleotide purity analysis in support of oligonucleotide production, purification and research. Compared to traditional methods, the Oligo PRO™ offers superior separation resolution, higher sample throughput, automation and improved quantification of oligonucleotide impurities.
Key Benefits:
- Parallel 96-capillary gel electrophoresis with on-line UV absorbance detection (CGE-UV) provides unsurpassed throughput and automation
- CGE-UV is the method of choice for assessing synthetic oligonucleotides purity, offering superior size-based resolution and nanoliter (nL) sampling volumes. Relative purity is determined on-line by directly measuring UV absorbance. Signal intensity is free from the sequence and size-dependent variations caused by ionization suppression that prove troublesome in mass spectrometry methods.
- Predefined separation and capillary conditioning methods, and an intuitive user interface help streamline instrument operation. Additionally, a lower cost, 24-capillary system is also available for medium throughput applications.
- Advanced data analysis software performs manual or automated peak integration and provides customized reports for individual samples or an entire 96-well sample plate.
- Single nucleotide resolution can be achieved for up to 100-mer lengths and higher using appropriate sample loading conditions.
- A unique, four position slide-out stage accommodates up to three 96-well sample plates allowing for unattended analysis of up to 288 samples.
- A full line of reagents, specifically optimized for use with the Oligo PRO™ system, provide a cost effective, "turn-key" solution for synthetic oligonucleotide purity analysis.
The above figure shows the separation of a mixture of synthetic DNA oligonucleotides analyzed on the Oligo PRO™ system. Single nucleotide resolution can be achieved across the entire size range using patented Oligel® gel matrix.
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